Production of colony-stimulating factor by leukemic leukocytes

Production of colony-stimulating factor by leukemic leukocytes.

The production of colony-stimulating factor (CSF) by the peripheral blood cells of untreated patients with acute myeloid leukemia (AML) was measured in the agar culture system using normal human bone marrow as the source of colony-forming units (CFUc). CSF production was found to be variable and was related to the morphologic subtype of AML--cells from patients with monocytic leukemia produced ...

THE ROLE OF ALVEOLAR MACROPHAGES IN THE PRODUCTION OF COLONY –STIMULATING FACTOR BY THE LUNG

The role of alveolar macrophages in the production of granulocyte/ macrophage colony-stimulating factor(s) by the rat lung was investigated. Lavaged lungs, when incubated at proper weight per volume of culture medium, produced the same amount of colony-stimulating factor as unlavaged ones. Both lavaged and unlavaged lungs produced similar types of colony-stimulating factor (s). Prolonged i...

Production of Recombinant Human Granulocyte-Colony Stimulating Factor by Pichia pastoris

Human granulocyte-colony stimulating factor (hG-CSF) cDNA was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase (AOX1) promoter. An expression vector for hG-CSF secretion was constructed using vector pPIC9. Higher levels of hG-CSF was obtained using a P. pastoris Mut+ (methanol utilization fast) phenotype. The effects of environmental factors such as...

THE EFFECT OF THEOPHYLLINE ON THE PRODUCTION OF COLONY STIMULATING FACTOR (CSF)BY THE LUNG

The effect of theophylline on colony stimulating factor production by the lung tissue was investigated. Addition of various concentrations of theophylline to the lung conditioned medium enhanced CSF production at 10 µg/ml and at higher concentrations showed an inhibitory effect. Determination of the phosphate content of the medium showed a decrease at 1-3 hours of incubation. Addition of A...

Responsiveness of human granulocytic leukemic cells to colony-stimulating factor.